Molecular cloning and sequence analysis of an andean potato mottle virus cDNA clone encoding the major coat protein.
نویسندگان
چکیده
Departamento de Genetica, Universidade Federal do Rio de Janeiro, CP 6801 1, CEP 21944, Rio de Janeiro, Brasil (N.S., R.K., D.E.d.O.); Departamento de Fitopatologia, Universidade Federal Rural do Rio de Janeiro, CP 23851, Rio de Janeiro, Brasil (P.S.T.B.); Departamento de Genetica, Fundagco Oswaldo Cruz, CP 926, Rio de laneiro, Brasil (A.C.P.V.); Departamento de Bioquimica, Universidade Federal do Rio de Janeiro, CP 1573, Rio de Janeiro, Brasil (D.B.F., D.E.d.0); and Laboratorium voor Genetica, Rijksuniversiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium (G.B.)
منابع مشابه
Molecular cloning of adenylate kinase from the human filarial parasite Onchocerca volvulus
Adenylate kinases (ADK) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. In this study, the cloning of a cDNA encoding an adenylate kinase from the filariaOnchocerca volvulus has been described. Using PCR technique, a 281 bp cDNA fragment encoding part ofan adenylate kinase was isolated from an O. volvulus cDNA library. Use of this fragment as a p...
متن کاملHeterologous Expression of Potato Virus Y Coat Protein, Isolate Pot187
Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
متن کاملCloning and Sequence Analysis of Gene Encoding OipA from Iranian Clinical Helicobacter pylori
Background: Outer inflammatory protein A (OipA) is one of the important adhesins of H. pylori and a valuable candidate for vaccine development. Its gene is under "on-off" switch status which correlates with OipA protein expression. Objectives: We aimed to obtain a recombinant OipA clone (with "on" status) from an Iranian clinical isolate. Materials and Methods: A clinical H. pylori-isolate demo...
متن کاملConstruction of Recombinant Bacmid DNA Encoding Newcastle Disease Virus (NDV) Fusion Protein Gene
Background and Aims: Newcastle disease virus (NDV) is one of the major pathogen in poultry. Vaccination is intended to control the disease as an effective solution nevertheless this virus is a growing threat to the poultry industry. F gene open reading frame (ORF) from NDV is 1650 bp, encoding a protein of 553 amino acids that can induce protective immunity alone. The F glycoprotein on the surf...
متن کاملSynthesis of an infectious full-length cDNA clone of rice yellow mottle virus and mutagenesis of the coat protein.
A full-length cDNA clone of rice yellow mottle sobemovirus (RYMV) was synthesized and placed adjacent to a bacteriophage T7 RNA polymerase promoter sequence. Capped-RNA transcripts produced in vitro were infectious when mechanically inoculated onto rice plants (Oryza sativa L). Individual full-length clones varied in their degree of infectivity but all were less infectious than native viral RNA...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 100 1 شماره
صفحات -
تاریخ انتشار 1992